Rapid Separation of Plasma Creatine Kinase Isoenzymes by Batch Adsorption on Glass Beads
نویسندگان
چکیده
منابع مشابه
Rapid separation of plasma creatine kinase isoenzymes by batch adsorption on glass beads.
To separate the MM and MB creatine kinase (EC 2.7.3.2) isoenzymes in small plasma samples, we developed a simple, rapid ion-exchange batch-adsorption procedure with "DEAE Glycophase" glass beads (Corning). All separative steps are performed in a single test tube and can be completed within a few minutes. Results of measurements of isoenzymes in plasma samples from patients with acute myocardial...
متن کاملImproved electrophoretic separation of creatine kinase isoenzymes.
Creatine kinase isoenzymes are separated electrophoretically on cellulose acetate, with use of an improved procedure for reactivation and visualization of enzymic activity. N-Acetyl cysteine is used as the reactivator and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide for visualization in a tetrazolium-coupled system. This test, both practical and reliable, is a suitable alter...
متن کاملIsoenzymes of creatine kinase; separation by acrylamide gel electrophoresis.
A method for the separation of creatine kinase isoenzymes is described utilizing vertical acrylamide slab electrophoresis. This technique combines the advantages of vertical separation methods with the greater resolving power of acrylamide. The inclusion of the coupling enzymes (hexokinase and glucose-6-phosphate dehydrogenase) in the buffer system should theoretically provide optimal concentra...
متن کاملChromatographic and electrophoretic separation of creatine kinase isoenzymes compared.
We compared two techniques for separating and evaluating serum creatine kinase isoenzymes--fluorometric agarose electrophoresis and Sephadex chromatography--in 50 patients, 25 of whom had confirmed acute myocardial infarction. In every case isoenzyme MB (heart isoenzyme) was detected with equal sensitivity by either procedure. Evidently, only the presence or absence of MB is clinically signific...
متن کاملAn interlaboratory study of creatine kinase and creatine kinase isoenzymes.
Over 400 laboratories participated in an interlaboratory study of creatine kinase (EC 2.7.3.2) in which they were requested to analyze five lyophilized samples for total creatine kinase and creatine kinase isoenzymes and to complete a questionnaire regarding methodology. The specimens were prepared from an homogenate of human myocardium that had been centrifuged, dialyzed, and added to a matrix...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1975
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/21.7.844